FIG 6.

Susceptibility of cells depleted of Cbl to virus superinfection. HEp-2 cells, the Cbl-knockdown derivatives, and control shRNA-treated cells were exposed to the wild-type virus at 30 PFU/cell. At 0 h, 2 h, 5 h, or 8 h postinfection, the cells were exposed to the EGFP-ICP0 virus (1 PFU/cell) for 2 h. Then the cells were harvested, total DNA was extracted as described in Materials and Methods, and quantification was done by qPCR analysis using primers for EGFP. Amplification of β-actin DNA served as an internal control for the normalization process.