FIG 7.

(A) Development of CIN85-depleted HEp-2 cells. HEp-2 cells were exposed to lentiviruses carrying different shRNAs against CIN85, which were propagated as described in Materials and Methods. After cells were selected in puromycin, the efficiency of depletion of CIN85 was evaluated by Western blot analysis using equal amounts of lysates from the shRNA-treated cell lines and the parental HEp-2 cells. Clone 22 demonstrated more efficient depletion of CIN85. β-Actin was used as a loading control. (B) The Cbl KD cells are more susceptible to virus superinfection than the CIN85 KD cells. HEp-2, the Cbl KD, CIN85 KD derivatives, and nontargeted shRNA-treated cells were exposed to the wild-type virus and the EGFP-ICP0 virus, as described in the legend of Fig. 6. The entry of the EGFP-ICP0 virus was quantified by qPCR as described in the legend of Fig. 6.