FIG 6.

Proposed model for Pns11 filament-mediated dissemination of RGDV into the apical plasmalemma-lined cavities in the salivary glands of R. dorsalis. Viruses first enter the cytoplasm of secretory cells and then initiate multiplication processes for the assembly of progeny virions. Some virions are associated with virus-induced Pns11 filaments in the cytoplasm. These virus-associated filaments induce an exocytosis-like process to pass through the apical plasmalemma into the salivary cavity, facilitating viral release from secretory cells and finally into the salivary duct. Arrows indicate the process of viral release from salivary glands. APL, apical plasmalemma; BL, basal lamina; Cv, cavity; N, nucleus; SC, salivary cytoplasm; SD, salivary duct; Vp, viroplasm.