TABLE 1.

Treatment with dsPns11 via microinjection suppressed viral release into PSG cavities of R. dorsalis and suppressed viral transmission from insect vectors to rice plants^e

Treatmenta	Mean no. (± SE) of PSGs with viral antigens and Pns11 filaments in the cytoplasm or cavity (n = 50)b		Transmission rate (%) of RGDV from individual dsGFP- or dsPns11-treated R. dorsalis to rice plantsc
	Cytoplasm (limited)	Cytoplasm and cavity (extensive)
dsGFP	42.3 ± 1.8	35 ± 2.1	65.8 ± 3.8
dsPns11	40 ± 1.5	15.3 ± 0.9	32.4 ± 2.8
P valued	0.374	0.001	0.004

^aNymphs of R. dorsalis at 8 days padp were microinjected with 200 nl dsGFP or dsPns11 (0.5 g/liter), and a single rice plant was fed to individual dsGFP- or dsPns11-treated R. dorsalis for 2 days.

^bOccurrence of RGDV antigens and Pns11 in PSGs of R. dorsalis was detected via immunofluorescence microscopy 3 days after microinjection.

^cTransmission rate is the number of positive rice plants/total number of survival rice plants fed by dsRNAs-treated leafhoppers.

^dP values were estimated by Tukey's honest significant difference (HSD) test (at P values of 0.05).

^eValues are from three replicates.