FIG 6.

Kinetic analysis of endogenous reverse transcription in purified HIV-1 cores. Endogenous reverse transcription was analyzed with gradient-purified wild-type HIV 1 cores. Reactions were initiated by the addition of dNTPs and MgCl₂ and incubated at 37°C for the indicated time periods, and the copy numbers of the minus-strand strong stop and first-strand transfer products were determined by qPCR. The efficiency of the viral cDNA synthesis was calculated as the percentage of viral RNA converted to the respective viral cDNA product. Similar results were obtained when reactions were performed at 25°C to simulate the reactions performed for AFM analysis.