TABLE 4.

CVB3 site-directed mutagenesis primers

Mutation	Primer	Sequence (5′-3′)	Length (nt)	Position (nt)	Tma
A239G	MFAT1	TTACTCTGGGTACGATGGTAGCTTAAGCCCTGTCTG	39	719	73.7
	MRAT1	CAGACAGGGCTTAAGCTACCATCGTACCCAGAGTAA	39	719	73.7
A372V	MFAT2	GTTACCTGGACCAACGTCACTTTCCTAAAGAGG	33	1118	69.5
	MRAT2	CCTCTTTAGGAAAGTGACGTTGGTCCAGGTAAC	33	1118	69.5
K159R	MFAT3	GGTGACTTATGTAAGAGATGAGCTCAGGTC	30	479	66.8
	MRAT3	GACCTGAGCTCATCTCTTACATAAGTCACC	30	479	66.8
K159 M	MFAT4	GGTGACTTATGTAATGGATGAGCTCAGGTC	30	479	66.8
	MRAT4	GACCTGAGCTCATCCATTACATAAGTCACC	30	479	66.8

^aT_m, melting temperature.