FIG 4.

Role of ORF7 in viral entry, genome replication, and expression of typical viral genes. ARPE-19 cells, dNPCs, and dSY5Y cells were infected with cell-free rOka, 7D, and 7R viruses, respectively, at an MOI of 0.001. The cells were harvested at 10 hpi. (A) Entry of the viruses. The harvested cells were fixed and subjected to gE IFA staining. The gE-positive cells are counted as infected cells with viral entry. (B) Viral genome replication. DNA was extracted from the cells infected with VZV and the mutants, and viral genome copy numbers were determined by qPCR. (C) Transcription of typical viral genes. Harvested cells were subjected to RNA extraction, followed by quantitation of the mRNA level of viral genes by qRT-PCR. (D) Expression of typical glycoproteins. Cell lysates were prepared from the harvested cells. Four representative glycoproteins and pORF7 were detected by Western blotting. All of the results were obtained from three independent experiments and are represented as averages ± the SD or else representative images are shown.