Fig. 8.

Making and visualizing a mouse forelimb atlas from confocal microscopy scans. a The original scan contains three channels of muscles (red), tendons (green), and nerves (blue). The scale bar represents 500 μm. b Using brush-based SOI selection and extraction, we segment 21 muscles of the paw. Each muscle is an independent channel with adjustable settings. c The 22 segmented muscles of the forearm. d The 22 segmented muscles around the shoulder area. e The 5 segmented spinal nerves going down to the forelimb. f The 17 segmented tendons of the forearm muscles. g We segment the anatomical structures of the forelimb into 91 channels. Each channel is assigned a color for visualization