Table 4.
Prediction of nsp1 to nsp16 and the cleavage sites of polyproteins 1a and 1b of the AcCoV-JC34
| nsp | First-last amino acid residues | Protein size (aa) | Cleavage site | Putative functional domain(s) |
|---|---|---|---|---|
| nsp1 | M1-G218 | 218 | SCPCG|KSAFT | Unknown |
| nsp2 | K219-G820 | 602 | WVCKCG|AEVQLS | Unknown |
| nsp3 | A821-A2277 | 1457 | TRVGTA|DLAVFN | ADRP, PL1pro, PL2pro |
| nsp4 | D2278-Q2779 | 502 | LNAQ|SCAK | Hydrophobic domain |
| nsp5 | S2780-Q3042 | 263 | AKVQ|IEGA | 3CLpro |
| nsp6 | I3043-Q3319 | 277 | SGMQ|CSWA | Hydrophobic domain |
| nsp7 | C3320-Q3437 | 118 | STIQ|SKLT | Unknown |
| nsp8 | S3438-Q3715 | 278 | VKLQ|NNEI | Unknown |
| nsp9 | N3716-Q3820 | 105 | VRLQ|AGKP | Unknown |
| nsp10 | A3821-Q3955 | 135 | STVQ|SNII | Unknown |
| nsp11 | S3956-N3974 | 19 | - | Short peptide at the end of ORF1a |
| nsp12 | L3956-Q4881 | 926 | SVLQ|SAGL | RdRp |
| nsp13 | S4882-Q5478 | 597 | TDLQ|SVLS | Hel |
| nsp14 | S5479-Q5998 | 520 | PILQ|GLEN | ExoN |
| nsp15 | G5999-Q6334 | 346 | PQLQ|NSEW | NendoU |
| nsp16 | N6345-K6646 | 302 | - | O-MT |
Superscript numbers indicate positions in polyprotein pp1ab, with the supposition of a ribosomal frame shift resulting in a peptide bond between N3974/L3975 for the expression of ORF1ab