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Metabolic activity of long-term cultured human PCLS. Metabolic activity in human PCLS was determined by the WST-1 assay. The negative control Triton X-100, a commonly used detergent, decreased metabolic activity in human PCLS to an OD corresponding to background (~0.04). Data are presented as Box and Whiskers, Mann-Whitney test (WST-1: n = 8(day 1, 8, 15, Triton), n = 5(day 5), n = 6(day 12, 14)). The dotted line marks the mean measured OD on day 1. PCLS = precision cut lung slices
