Table 2.
Antigen | Description of Immunogen | Source, Host Species, Cat. #, Clone or Lot#, RRID | Concentration Used |
---|---|---|---|
Glutamic acid decarboxylase 67 (GAD67) | Recombinant whole protein of rat GAD67. | Millipore, mouse monoclonal, Cat# MAB5406, RRID: AB_2278725. | 1:1000 |
MAP2 | Bovine brain microtubule protein (aa 997–1332). | Millipore, mouse monoclonal clone AP20, Cat# MAB3418, RRID: AB_94856. | 1:200 |
NeuN | Purified cell nuclei from mouse brain. | Millipore, mouse monoclonal clone A60, Cat# MAB377, RRID: AB_94966. | 1:100 |
Vesicular glutamate transporter 2 (VGLUT2) | KLH-conjugated, synthetic peptide corresponding to amino acids residues CWPNGWEKKEEFVQESAQDAYS YKDRDDYS of rat VGLUT2 (aa. 554–582). |
Professor Takeshi Kaneko (Kyoto University), guinea-pig and rabbit polyclonal. | 0.5 μg/ml |
Green fluorescent protein (GFP) | Recombinant whole protein of enhanced green fluorescent protein. | Professor Takeshi Kaneko (Kyoto University), guinea-pig and rabbit polyclonal. | 0.2 μg/ml |
Monomeric red fluorescent protein (mRFP) | Recombinant whole protein of red fluorescent protein. | Professor Takeshi Kaneko (Kyoto University), guinea-pig and rabbit polyclonal. | 0.2 μg/ml |