Fig. 4. Expression of eotaxin, IL-5, IL-13, and serum OVA-pecific IgE. Intestinal tissue was homogenized in 2.0 mL of PBS (pH 7.4) and supernatants assayed by ELISA for eotaxin-1 (A), IL-5 (B), and IL-13 (C). Intestinal tissue protein levels were quantitated using a BCA protein assay (Pierce, Rockford, IL, USA). Results are expressed as pg/mg intestinal mucosa protein. Serum OVA-specific IgE levels (D) were measured by ELISA on day 39 in different groups of mice. Data are expressed as means±SE. IL, interleukin; OVA, ovalbumin; IgE, immunoglobulin E; PBS, phosphate buffered saline; ELISA, enzyme-linked immunosorbent assay; SE, standard error. ^*P<0.05; ^†P<0.005.