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. 2017 May 16;19(7):1479–1493. doi: 10.1016/j.celrep.2017.04.062

Figure 3.

Figure 3

EBV Lytic Replication Targets the BCR Complex for Degradation

(A) Immunoblot of whole-cell extracts indicate downregulation of IgM and CD79A in gp350+ ZHT/RHT cells, representative of three independent experiments.

(B and C) Downregulation of IgM during P3HR1 lytic EBV replication confirmed by flow cytometry. Shown are representative plots for three independent experiments of uninduced (B; left) and 48-hr 4-HT-induced P3HR1 (B; right) or of uninduced (C; left) and 48 hr 12-O-Tetradecanoylphorbol-13-Acetate (TPA)/butyrate-induced P3HR1 (C; right).

(D) EBV lytic replication destabilizes the IgG BCR complex. EBV+ Akata B cells were induced to undergo lytic replication by TPA/butyrate treatment for 24 hr. WCL from sorted gp350+ cells were immunoblotted are as indicated.