Table S3.

Score for skin irritation study on normal rabbit skin calculated after 3 days of administration and 3 days of observation

Category for reaction	1.5 mg/mL CAP solution	0.75 mg/mL CAP solution	1.5 mg/mL CAP-loaded NLCs	1.5 mg/mL CAP-loaded NLCs gel
1 h after first administration	2	2	1	0
Before second administration	1	1	0	0
1 h after second administration	2	1	1	1
Before third administration	1	1	0	0
1 h after third administration	2	2	1	1
24 h after third administration	2	1	0	0
48 h after third administration	0	0	0	0
72 h after third administration	0	0	0	0

Note: 0–0.49: non-irritation, 0.5–2.99: slight irritation, 3.0–5.99: moderate irritation, 6.0–8.0: strong irritation.

Abbreviations: CAP, capsaicin; NLCs, nanolipoidal carriers.