Figure 4.

Natural killer (NK) hypomaturation profile is associated low frequency of memory-like NK cells. NK alterations associated with the different maturation phenotypes were explored by mass cytometry. Peripheral blood mononuclear cells from 17 additional patients with newly diagnosed acute myeloid leukemia and 7 healthy volunteers (HVs) were analyzed by mass cytometry. Memory-like NK cells were defined as CD56^dim/CD57⁺/NKG2C⁺ NK cells. (A) Analysis of the percentage of memory-like NK cells by NK maturation group. (B) Gating strategy of memory-like NK cells on a Spade tree. (C) Representative Spade trees of patients and HVs. The first column represents CD56 expression, which defines the cluster of CD56^bright NK cells. The second column displays CD57 expression, which divides CD56^dim NK cells into CD57⁺ and CD57⁻ NK cells. The third column displays NKG2C expression, which enables to identify the cluster of memory-like NK cells, defined as CD56^dim/CD57⁺/NKG2C⁺ NK cells.