Table 1.

Sequences of amino acids near and in TM2 transmembrane regions of 28 nAChR subunits.

sp.	sub.	Uniprot			TM2
			{−5′− 4′}	{−3′ 0′}	{1′ 18′}	{19′}	{20′}	{21′ 24′}	{25′ 29′}
H	α1	P02708	$\text{D}$S	_G$\text{E}$$\text{K}$	MTLSISVLLSLTVFLLVI	V	$\text{E}$	LIPS	TSSAV
H	β1	P11230	$\text{D}$A	_G$\text{E}$$\text{K}$	MGLSIFALLTLTVFLLLL	A	$\text{D}$	$\text{K}$VP$\text{E}$	TSLSV
H	γ	P07510	$\text{K}$A	GGQ$\text{K}$	CTVAINVLLAQTVFLFLV	A	$\text{K}$	$\text{K}$VP$\text{E}$	TSQAV
H	δ	Q07001	$\text{D}$S	_G$\text{E}$$\text{K}$	TSVAISVLLAQSVFLLLI	S	$\text{K}$	RLPA	TSMAI
H	ε	Q04844	QA	GGQ$\text{K}$	CTVSINVLLAQTVFLFLI	A	Q	$\text{K}$IP$\text{E}$	TSLSV
M	α1	P04756	$\text{D}$S	_G$\text{E}$$\text{K}$	MTLSISVLLSLTVFLLVI	V	$\text{E}$	LIPS	TSSAV
M	β1	P09690	$\text{D}$A	_G$\text{E}$$\text{K}$	MGLSIFALLTLTVFLLLL	A	$\text{D}$	$\text{K}$VP$\text{E}$	TSLAV
M	γ	P04760	$\text{K}$A	GGQ$\text{K}$	CTVATNVLLAQTVFLFLV	A	$\text{K}$	$\text{K}$VP$\text{E}$	TSQAV
M	δ	P02716	$\text{D}$C	_G$\text{E}$$\text{K}$	TSVAISVLLAQSVFLLLI	S	$\text{K}$	RLPA	TSMAI
M	ε	P20782	QA	GGQ$\text{K}$	CTVSINVLLAQTVFLFLI	A	Q	$\text{K}$IP$\text{E}$	TSLSV
H	α2	Q15822	$\text{D}$C	_G$\text{E}$$\text{K}$	ITLCISVLLSLTVFLLLI	T	$\text{E}$	IIPS	TSLVI
H	α3	P32297	$\text{D}$C	_G$\text{E}$$\text{K}$	VTLCISVLLSLTVFLLVI	T	$\text{E}$	TIPS	TSLVI
H	α4	P43681	$\text{E}$C	_G$\text{E}$$\text{K}$	ITLCISVLLSLTVFLLLI	T	$\text{E}$	IIPS	TSLVI
H	α5	P30532	N$\text{E}$	_G$\text{E}$$\text{K}$	ICLCTSVLVSLTVFLLVI	$\text{E}$	$\text{E}$	IIPS	SS$\text{K}$VI
H	α6	Q15825	$\text{D}$C	_G$\text{E}$$\text{K}$	VTLCISVLLSLTVFLLVI	T	$\text{E}$	TIPS	TSLVV
H	α7	P36544	$\text{D}$S	_G$\text{E}$$\text{K}$	ISLGITVLLSLTVFMLLV	A	$\text{E}$	IMPA	TS$\text{D}$SV
H	β2	P17787	$\text{D}$C	_G$\text{E}$$\text{K}$	MTLCISVLLALTVFLLLI	S	$\text{K}$	IVPP	TSL$\text{D}$V
H	β4	P30926	$\text{D}$C	_G$\text{E}$$\text{K}$	MTLCISVLLALTFFLLLI	S	$\text{K}$	IVPP	TSL$\text{D}$V
M	α4	O70174	$\text{E}$C	_G$\text{E}$$\text{K}$	VTLCISVLLSLTVFLLLI	T	$\text{E}$	IIPS	TSLVI
M	α5	Q2MKA5	N$\text{E}$	_G$\text{E}$$\text{K}$	ISLCTSVLVSLTVFLLVI	$\text{E}$	$\text{E}$	IIPS	SS$\text{K}$VI
M	β2	Q9ERK7	$\text{D}$C	_G$\text{E}$$\text{K}$	MTLCISVLLALTVFLLLI	S	$\text{K}$	IVPP	TSL$\text{D}$V
R	α7	Q05941	$\text{D}$S	_G$\text{E}$$\text{K}$	ISLGITVLLSLTVFMLLV	A	$\text{E}$	IMPA	TS$\text{D}$SV
R	α9	P43144	AS	_G$\text{E}$$\text{K}$	VSLGVTILLAMTVFQLMV	A	$\text{E}$	IMPA	S$\text{E}$NVP
R	α10	Q9JLB5	$\text{D}$S	_G$\text{E}$$\text{K}$	VSLGVTVLLALTVFQLIL	A	$\text{E}$	SMPP	A$\text{E}$SVP
C	α3	P09481	$\text{D}$C	_G$\text{E}$$\text{K}$	VTLCISVLLSLTVFLLVI	T	$\text{E}$	TIPS	TSLVI
C	α4	P09482	$\text{E}$C	_G$\text{E}$$\text{K}$	ITLCISVLLSLTVFLLLI	T	$\text{E}$	IIPS	TSLVI
C	β2	P09484	$\text{D}$C	_G$\text{E}$$\text{K}$	MTLCISVLLALTVFLLLI	S	$\text{K}$	IVPP	TSL$\text{D}$V
C	β4	P26153	$\text{D}$C	_G$\text{E}$$\text{K}$	MTLCISVLLALTVFLLLI	S	$\text{K}$	IVPP	TSL$\text{D}$V

Negative amino acid residues are reported in red, positive ones in green. H, human; M, mouse; R, rat; C, chicken. In brackets the positions of amino acid residues according to Miller (1989).