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. 2017 May 29;8:615. doi: 10.3389/fimmu.2017.00615

Figure 2.

Figure 2

Myeloid differentiation factor 88 (MyD88) knockdown blocks the induction of macrophage CD80 expression without affecting HLA-DR in response to Toll-like receptor (TLR) ligands. Control (shCnt) vs. shMyD88 transduced THP-1 cells were differentiated for 72 h with PMA, with the addition of 100 ng/mL Pam3csk4, 20 ng/mL IFNγ, or 100 ng/mL Pam3csk4 + 20 ng/mL IFNγ. For the final 24 h. Cells were then analyzed for CD80 (A–C) and HLA-DR (D–F) expression by flow cytometry. Data in panels (B,C,E,F) are presented as % difference in intensity between each group (ΔMFI). Representative histograms (A,D) and combined data of triplicates from two independent experiments are shown (B,C,E,F). The two-tailed t-test was used for statistical evaluation.