Skip to main content
. 2017 May 29;8:615. doi: 10.3389/fimmu.2017.00615

Figure 5.

Figure 5

Inhibition of myeloid differentiation factor 88 in murine and human T cells reduces IFNγ production. (A) Primary human CD4+ T cells were isolated from buffy coats and stimulated with IL-18 + IL-12 (320 and 160 ng/mL, respectively) overnight in the absence or presence of MyDI or MyDI-sc (20 µM/mL). The results are the average of triplicates and one representitve experiment of three independent experiments is shown. (B) Murine MOG35-55 specific T cells were incubated with MyDI or MyDI-sc (20 µM/mL) or without peptide for 2 h. T-cells were then plated with or without 30 ng/mL IL-18 ON and IFN-γ production was measured by ELISA. (C) MOG35-55 specific T cells were incubated with MyDI or MyDI-sc, or without peptide for 2 h. T-cells were then plated with or without 30 ng/mL IL-18 ON in the presence of irradiated splenocytes and MOG35-55 peptide (5 µg/mL). The results are average of triplicates and representitve of two independent experiments. The two-tailed t-test was used for statistical evaluation.