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. 2017 May 29;8:606. doi: 10.3389/fimmu.2017.00606

Figure 4.

Figure 4

Dose assays of N. caninum tachyzoites-induced neutrophil extracellular trap formation. Bovine polymorphonuclear neutrophil (PMN) and N. caninum tachyzoites were incubated at different ratios (PMN: tachyzoites = 1:1, 1:2, 1:3, 1:4). After incubation (120 min), samples were analyzed for extracellular DNA by quantifying Pico Green®-derived fluorescence intensities. Each condition was performed in duplicates. Arithmetic means of three PMN donors, minimum, and maximum. Differences were regarded as significant at a level of *p ≤ 0.05 and **p ≤ 0.01.