Figure 4.

GbaVd1 and GbaVd2 conferred Verticillium wilt resistance in transgenic Arabidopsis. (A) Identification of Verticillium wilt resistance after interfamily transfer of GbaVd1 and GbaVd2 in Arabidopsis. Four-week-old seedlings of homozygote transgenic Arabidopsis (T₃) were inoculated with 5 mL of conidial suspension (5 × 10⁶ conidia/mL). The Verticillium wilt phenotypes were determined and photographed 4weeks after inoculation. WT, the wild-type Col-0; Mock, inoculation with sterile water; OE^GbaVd1/OE^GbaVd2, transgenic plants overexpressing GbaVd1 or GbaVd2, respectively. (B) Real-time PCR quantification of fungal biomass in GbaVd1 or GbaVd2 transgenic plants. Error bars represent standard errors, ^**significant differences (P ≤ 0.01), according to unpaired Student's t-test. (C) Investigation of the inflorescence height after inoculation with V. dahliae. Error bars represent standard errors, ^**significant differences (P ≤ 0.01), according to unpaired Student's t-test.