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. 2017 May 29;8:894. doi: 10.3389/fpls.2017.00894

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Copyright © 2017 Brocard, Immel, Coulon, Esnay, Tuphile, Pascal, Claverol, Fouillen, Bessoule and Bréhélin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of AtULP and AtSRP1-fluorescent fusions in lipid droplets. Arabidopsis cotyledons (A,C–E), or N. benthamiana leaves (B) expressing TagRFP-AtULP (A,B) AtSRP1-YFP (B), AtSRP1-TagRFP (C–E), or YFP-AtULP (E) were visualized by confocal laser scanning microscopy. Arabidopsis plantlets were co-labeled with neutral lipid specific dye BODIPY^493/503(A,C,D). Co-labeling of AtULP or AtSRP1 targeted structures by BODIPY^493/503 confirmed that these structures are lipid droplets. Bodipy, chlorophyll, and trans indicate Bodipy fluorescence, chlorophyll autofluorescence and transmission microscopy image respectively. Merge indicates merge of TagRFP, Bodipy and chlorophyll fluorescences (A,C,D) or YFP and TagRFP fluorescence (B,E). Bar: 5 μm. White arrows indicate specific localization of AtULP in cells expressing both AtSRP1 and AtULP transgenes.