Table 2.
Protein abundance in aging leaf LD determined by TOP3 method.
| UniProtKB IDb | GENE IDc | Protein named | Abundance in LD estimated by TOP3 method | Average Abundancee | Average Protein Mass contribution (%)f | ||
|---|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp. 3 | |||||
| KNOWN LIPID DROPLET PROTEINSa | |||||||
| PXG3_ARATH | AT2G33380 | Caleosin-related family protein (CLO3/RD20) | 1.16E+08 | 2.35E+07 | 1.62E+07 | 5.20E+07 | 50.18 |
| DOX1_ARATH | AT3G01420 | Peroxidase superfamily protein (α-DOX1) | 5.50E+05 | 2.30E+05 | 4.47E+04 | 2.75E+05 | 0.28 |
| Y1736_ARATH | AT1G67360 | Rubber elongation factor protein (SRPP1/LDAP1) | 7.79E+07 | 7.87E+06 | 7.20E+06 | 3.10E+07 | 23.86 |
| BIOSYNTHESIS OF SECONDARY METABOLITES | |||||||
| Q9C9C2_ARATH | AT1G74010 | Strictosidine synthase 2 (STR2) | 5.18E+06 | 9.02E+05 | 6.55E+05 | 2.25E+06 | 2.06 |
| SSL12_ARATH | AT1G74020 | ||||||
| PCYOX_ARATH | AT5G63910 | Farnesylcysteine lyase (FCLY) | 2.62E+05 | 2.83E+05 | nd | 2.05E+05 | 0.31 |
| GSL_ARATH | AT2G25450 | 2-Oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein (GSL-OH) | 4.57E+05 | 2.06E+06 | nd | 1.26E+06 | 2.24 |
| NSP5_ARATH | AT5G48180 | Nitrile specifier protein 5 (NSP5) | 9.26E+04 | 1.98E+05 | nd | 1.45E+05 | 0.23 |
| C89A9_ARATH | AT3G03470 | Cytochrome P450. family 87. subfamily A | 7.30E+05 | 4.58E+05 | nd | 5.94E+05 | 0.62 |
| CLH1_ARATH | AT1G19670 | Chlorophyllase 1 | 1.94E+06 | 1.15E+06 | 2.94E+05 | 1.13E+06 | 1.39 |
| BIOSYNTHESIS OF AMINO ACIDS | |||||||
| METK3_ARATH | AT2G36880 | Methionine adenosyltransferase 3 (SAMS3) | 1.97E+06 | 3.28E+05 | 2.05E+05 | 8.35E+05 | 0.72 |
| Q9SK84_ARATH | AT1G22410 | 3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase (DHAP) | 1.47E+06 | 2.56E+05 | 5.09E+04 | 5.92E+05 | 0.43 |
| AROG_ARATH | AT4G33510 | ||||||
| STRESS RESPONSE | |||||||
| ERD7_ARATH | AT2G17840 | Early-responsive to dehydration 7 (ERD7) | 5.92E+05 | 3.32E+05 | 3.52E+04 | 3.20E+05 | 0.35 |
| PER70_ARATH | AT5G64110 | Peroxidase superfamily protein | 4.31E+05 | nd | 5.16E+04 | 2.41E+05 | 0.17 |
| EXOL4_ARATH | AT5G09440 | EXORDIUM like 4 | nd | 2.85E+05 | 1.54E+05 | 2.20E+05 | 0.57 |
| CELL WALL MODIFICATION | |||||||
| XTH4_ARATH | AT2G06850 | Xyloglucan endotransglucosylase/hydrolase 4 | nd | 9.88E+05 | 2.29E+05 | 6.08E+05 | 1.43 |
| XTH16_ARATH | AT3G23730 | Probable xyloglucan endotransglucosylase/hydrolase protein 16 | |||||
| XTH15_ARATH | AT4G14130 | Xyloglucan endotransglucosylase/hydrolase protein 15 | |||||
| XTH5_ARATH | AT5G13870 | Probable xyloglucan endotransglucosylase/hydrolase protein 5 | |||||
| PEPTIDASES | |||||||
| Q0WVJ5_ARATH | AT1G09850 | Xylem bark cysteine peptidase 3 | 1.51E+06 | nd | 7.19E+04 | 7.89E+05 | 0.42 |
| Q9SUL1_ARATH | AT4G16190 | Papain family cysteine protease | 4.49E+05 | nd | 7.18E+04 | 2.60E+05 | 0.21 |
| CYTOSKELETON PROTEINS | |||||||
| ACT8_ARATH | AT1G49240 | Actin | 3.06E+07 | 6.72E+06 | 2.66E+06 | 1.33E+07 | 11.71 |
| ACT2_ARATH | AT3G18780 | Actin 2 | |||||
| TBB6_ARATH | AT5G12250 | Beta-6 tubulin | 1.52E+06 | nd | 1.23E+05 | 8.20E+05 | 0.51 |
| TBB5_ARATH | AT1G20010 | Tubulin beta-5 chain | 5.17E+06 | 5.52E+05 | 2.00E+05 | 1.98E+06 | 1.28 |
| TBB1_ARATH | AT1G75780 | Tubulin beta-1 chain | |||||
| TRANSPORT | |||||||
| Intracellular lipid transport | |||||||
| TGD2_ARATH | AT3G20320 | Trigalactosyldiacylglycerol2 (TGD2) | 6.67E+05 | 2.31E+05 | 4.06E+04 | 3.13E+05 | 0.29 |
| Ion transmembrane transporter | |||||||
| VATL2_ARATH | AT1G19910 | ATPase, F0/V0 complex, subunit C protein | 2.12E+05 | 1.09E+05 | 0.12 | ||
| VATL4_ARATH | AT1G75630 | Vacuolar H+-pumping ATPase | 9.45E+04 | 1.90E+04 | |||
| VATL5_ARATH | AT2G16510 | V-type proton ATPase | |||||
| ACA4_ARATH | AT2G41560 | Autoinhibited Ca(2+)-ATPase, isoform 4 | 3.32E+05 | 1.48E+05 | 7.60E+03 | 1.62E+05 | 0.15 |
| Intracellular protein transport | |||||||
| SNAA2_ARATH | AT3G56190 | Alpha-soluble NSF attachment protein 2 | 9.64E+05 | 2.55E+05 | 6.13E+04 | 4.27E+05 | 0.37 |
| SNAA1_ARATH | AT3G56450 | Alpha-soluble NSF attachment protein 1 | |||||
| NSF_ARATH | AT4G04910 | N-ethylmaleimide sensitive factor | 2.12E+05 | 9.45E+04 | 1.90E+04 | 1.09E+05 | 0.12 |
| AB2E_ARATH | AT4G19210 | ATP-Binding Cassette E2 | 3.26E+05 | 6.02E+04 | nd | 1.93E+05 | 0.13 |
| MISCELLANEOUS | |||||||
| Q94EY7_ARATH | AT5G16550 | Unknown protein | 8.31E+06 | 6.93E+05 | 3.40E+05 | 3.12E+06 | 1.95 |
| Q9FNC4_ARATH | AT5G44020 | HAD superfamily, subfamily IIIB acid phosphatase | nd | 1.21E+05 | nd | 1.21E+05 | 0.25 |
a
Biological process according to MapMan. UniProt Gene Ontology and KEGG BRITE database.
b
Protein UniProt identifier.
c
Gene identifier from TAIR database.
d
Protein name according to TAIR database.
e
Average of normalized abundance in LD estimated by TOP3 method for the three experiments.
f
Average of protein mass contribution in LD (in percent).