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. 2017 May 29;7:204. doi: 10.3389/fcimb.2017.00204

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Copyright © 2017 Guo, Hall, Yang and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of coa heteroexpression or addition of recombinant coagulase on bacterial survival. (A) Percent survival and (B) bacterial load of wild-type S. epidermidis and coa expression in trans in human blood during induction. (C) SDS-PAGE analysis of purified recombinant Coa (coagulase) and control protein SarZ from E. coli. M, ColorPlus Prestained protein Marker (NEB), the unit of size is kDa. (D) Coagulation activity of purified recombinant Coa (rCoa) from E. coli in human blood. CT, negative control without addition of rCoa. (E) Percent survival of wild-type USA300 CA-MRSA 923 and addition of different concentrations of purified rCoa and control rSarZ protein in human blood. Data is the mean and standard error of at least four experiments per strain.