Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | References |
|---|---|---|
| DC10B | Dam− E. coli | Monk et al., 2012 |
| BL21 | Recombinant protein expression strain E. coli | |
| WCUH29 | Human clinical MRSA isolate, sigB+/rsbU+ | NCIMB40771; Hall et al., 2017 |
| WCUH29/pYH4 | WCUH29 with empty pYH4; ErmR | Sun et al., 2005 |
| Sa371 | WCUH29 saeS allelic replacement mutant with tetA gene; TcR | Liang et al., 2006 |
| Sa371/pYH4 | WCUH29 saeS::tetA with pYH4; TcR, ErmR | Liang et al., 2006 |
| Sa371com | WCUH29 saeS::tetA with pYH4-saeS; TcR, ErmR | Liang et al., 2006 |
| Sa371/pYH4-sa1000 | WCUH29 saeS::tetA with pYH4-sa1000; TcR, ErmR | This study |
| Sa371/pYH4-efb | WCUH29 saeS::tetA with pYH4-efb; TcR, ErmR | This study |
| Sa371/pYH4-fnbAB | WCUH29 saeS::tetA with pYH4-fnbAB; TcR, ErmR | This study |
| Sa371/pYH4-coa | WCUH29 saeS::tetA with pYH4-coa; TcR, ErmR | This study |
| WCUH29Δcoa | WCUH29 coa deletion mutant | This study |
| Sa371Δcoa | WCUH29 saeS and coa double mutant; TcR | This study |
| WCUH29Δcoa/pYH4 | WCUH29 coa deletion mutant with pYH4; ErmR | This study |
| WCUH29Δcoa/pYH4-coa | WCUH29 coa deletion mutant with pYH4-coa; ErmR | This study |
| Sa371Δcoa/pYH4 | WCUH29 coa deletion mutant with pYH4; TcR, ErmR | This study |
| Sa371Δcoa/pYH4-coa | WCUH29 saeRS and coa double mutant with pYH4-coa; TcR, ErmR | This study |
| Sa371Δcoa/pYH4-saeRS | WCUH29 saeRS and coa double mutant with pYH4-saeRS; TcR, ErmR | This study |
| 923 | USA300 CA-MRSA | Montgomery et al., 2010 |
| 923ΔsaeRS | 923 saeRS deletion mutant | This study |
| 923Δcoa | 923 coa deletion mutant | This study |
| 923ΔsaeRSΔcoa | 923 saeRS and coa double deletion mutant | This study |
| 923/pYH4 | 923 with pYH4; ErmR | This study |
| 923ΔsaeRS/pYH4 | 923 saeRS deletion mutant with pYH4; ErmR | This study |
| 923ΔsaeRS/pYH4-saeRS | 923 saeRS deletion mutant with pYH4-saeRS; ErmR | This study |
| 923Δcoa/pYH4 | 923 coa deletion mutant with pYH4; ErmR | This study |
| 923Δcoa/pYH4-coa | 923 coa deletion mutant with pYH4-coa; ErmR | This study |
| 923ΔsaeRSΔcoa/pYH4 | 923 saeRS and coa double deletion mutant with pYH4; ErmR | This study |
| 923ΔsaeRSΔcoa/pYH4-coa | 923 saeRS and coa double deletion mutant with pYH4-coa; ErmR | This study |
| 923ΔsaeRSΔcoa/pYH4-saeRS | 923 saeRS and coa double deletion mutant with pYH4-saeRS; ErmR | This study |
| MW2 | USA400 CA-MRSA human clinical isolate | Herold et al., 1998 |
| NW2saeRS::spec | MW2 saeRS allelic replacement with spectinomycin resistant gene; SpecR | Voyich et al., 2009 |
| NW2saeRS::spec/pYH4 | NW2 saeRS::spec with pYH4; ErmR, SpecR | This study |
| NW2saeRS::spec/pYH4-saeRS | NW2 saeRS::spec with pYH4-saeRS; ErmR, SpecR | This study |
| MW2Δcoa | MW2 coa deletion mutant | This study |
| MW2saeRS::specΔcoa | MW2 saeRS and coa double mutant; SpecR | This study |
| MW2/pYH4 | MW2 with pYH4; ErmR | This study |
| MW2Δcoa/pYH4 | MW2 coa deletion mutant with pYH4; ErmR | This study |
| MW2Δcoa/pYH4-coa | MW2 coa deletion mutant with pYH4-coa | This study |
| MW2saeRS::specΔcoa/pYH4 | MW2 saeRS and coa double mutant with pYH4; ErmR, SpecR | This study |
| MW2saeRS::specΔcoa/pYH4-coa | MW2 saeRS and coa double mutant with pYH4-coa; ErmR, SpecR | This study |
| MW2saeRS::specΔcoa/pYH4-saeRS | MW2 saeRS and coa double mutant with pYH4-saeRS, ErmR, SpecR | This study |
| S. epidermidis | Coagulase negative | Microbiology teaching lab |
| S. epidermidis/pYH4 | S. epidermidis with pYH4; ErmR | This study |
| S. epidermidis/pYH4-coa | S. epidermidis with pYH4-coa; ErmR | This study |
| PLASMIDS | ||
| pYH4 | Shuttle vector with Tc inducible promoter; ErmR | Huang et al., 2004 |
| pYH4-sa1000 | sa1000 cloned downstream of pYH4 tet promoter; ErmR | Liang et al., 2006 |
| pYH4-efb | efb(sa1003) cloned downstream of pYH4 tet promoter; ErmR | Liang et al., 2006 |
| pYH4-coa | coa cloned downstream of pYH4 tet promoter; ErmR | This study |
| pYH4-fnbAB | fnbAB cloned downstream of pYH4 tet promoter; ErmR | This study |
| pYH4-saeS | saeS cloned downstream of pYH4 tet promoter; ErmR | Liang et al., 2006 |
| pYH4-saeRS | saeRS cloned downstream of pYH4 tet promoter; ErmR | This study |
| pKOR1 | Temperature sensitive inducible allelic exchange plasmid for S. aureus; CmR | Bae and Schneewind, 2006 |
| pKOR1-coa | pKOR1 with in-frame coa upstream/downstream deletion region; CmR | This study |
| pKOR1-saeRS | pKOR1 with in-frame saeRS upstream/downstream deletion region; CmR | This study |
| pET24b | ||
| pET24b-sarZ | saZ cloned his-tag expression vector PET24b | Liang et al., 2011 |
| pET24b-coa | coa cloned his-tag expression vector PET24b | This study |