Table 2.
Oligonucleotides used in this study.
| Primer | Sequence (5′–3′) |
|---|---|
| saeRS-KO-pKOR1-L-F | CACGATCAGTAAGTGGGTCAT |
| saeRS-KO-pKOR1-L-R | GTAACATTACACAAATTAGACATTACGTCATAATC |
| saeRS-KO-pKOR1-R-F | GGGGACCACTTTGTACAAGAAAGCTGG GTGATGATGGAAGTACGGATACCAC |
| saeRS-KO-pKOR1-R-R | CGCACTCGAGTGACGTAATGTCTAATTTGTG |
| saeRfor1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCGGTAAAGAAATCGCAATGGTTG |
| saeSrev | AAACTATGACCCACTTACTGATCG |
| saeSfor1 | AAGCTAGCATGGTGTTATCAATTAGAAGTCAAATC |
| CoaRFor | ATTTATAACTCTATCCAAAGACATACAGTCAATAC |
| CoaRRevAttB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTGCCTATGACGCACAACGTGATGGTCG |
| CoaLForAttB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACATGAAGCAAAACTTGTATCCTTAGACG |
| CoaLRev | AATTTTTTAATTCCTCCAAAATGTAATTGCCC |
| Sa0222-for | GTTGTGTTGTTTCTTCAGCTTTACCAG |
| Sa0222-rev | CATGCCACACCATATTCTTCTCC |
| Coa-For-RBS | AGGAGGTTTAAACTATGAAAAAGCAAATAATTTCGCTAGGC |
| Coa-Rev-AscI | TTGGCGCGCCTTATTTTGTTACTCTAGGCCCATATGTCG |
| coaBamHIFor | TTTGGATCCATGAAAAAGCAAATAATTTCGCTAG |
| coaXholRev | CCGCTCGAGTTTTGTTACTCTAGGCCCATATG |
| FnbAB-For-RBS | AGGAGGTTTAAACT GTGAAAAACAATCTTAGGTACGGC |
| FnbAB-Rev-AscI | TTGGCGCGCCTTATGCTTTGTTATTCTTTTTATTTCTGCG |
| T7promoterfor | GATCTAAGCTTCGGGAATTCACTA G |
| T7terminatorrev | CAATACAATGTAGGCTGC |