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. 2017 May 29;8:307. doi: 10.3389/fphar.2017.00307

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Copyright © 2017 Hewitson, Holt, Tan, Wigg, Samuel and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pooled analyses show no overall effect of TGF-β1 on H3K9 mark enrichment in (myo)fibroblasts and NRK-52e. (A) Western blotting of duplicate samples from fibroblasts and NRK-52e with and without 48 h of TGF-β1 stimulation. Parallel blots were probed for H3K9Ac and H3K9Me3. Each blot was stripped and re-probed for H3 to correct for loading. (B) Summary of flow cytometric analysis presented as mean fluorescent intensity (MFI) of triplicate experiments in (B) fibroblasts and (C) NRK-52e. Bars represent mean ± SD from three independent experiments. ^∗p < 0.05, ^∗∗∗p < 0.001 vs. control; ns, not significant.