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. 2017 May 29;8:307. doi: 10.3389/fphar.2017.00307

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Copyright © 2017 Hewitson, Holt, Tan, Wigg, Samuel and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of TGF-β1 on the sub-nuclear distribution of histone marks. (A) Confocal images of immunofluorescently stained nuclei from fibroblasts and NRK-52e incubated with and without exogenous TGF-β1. Merged confocal micrographs show relative positioning of histone marks (H3K9Me3, H3K9Ac) and the nuclear envelope protein nucleoporin 62 (NUP62) in cells grown in control media, and media supplemented with TGF-β1. Scale bars = 10 μm. (B) Co-localisation of staining intensity for H3K9Me3 and NUP62 at a central nuclear plane (yellow line) in a nucleus from each experimental group.