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. 2017 May 24;10(4):491–500. doi: 10.1016/j.tranon.2017.04.002

Figure 5.

Figure 5

Effect of Chk1 inhibitors on radiation-induced double-strand breaks.

(A and B) Cells were X-irradiated at 1 Gy and incubated with vehicle (black), 5 nM UCN-01 (gray), or 200 nM MK-8776 (white) for the indicated times. The formation of double-strand breaks (DSBs) after X-irradiation was determined using a 53BP1 foci formation assay. Each graph represents the kinetics of 53BP1 focal formation after irradiation in EMT6 (A) or HeLa cells (B), in the presence or absence of Chk1 inhibitors. (C) EMT6 cells were X-irradiated at 2.5 Gy and incubated with vehicle (black) or MK-8776 (white) for the indicated times. The number of 53BP1 foci was evaluated. (D) EMT6 cells were X-irradiated at 1 or 2.5 Gy and incubated with vehicle (black) or MK-8776 (white) for the indicated times. The number of γ-H2AX foci was evaluated. At least 100 cells were analyzed and the average number of 53BP1 or γ-H2AX foci per cell was scored. Data are expressed as means ± S.D. from three experiments.