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Dexmedetomidine decreases the expression of NR2B, NF-κB and iNOS following CCI. The total RNA was extracted from spinal cord tissues using RNAiso kit. RT-PCR quantitation of NR2B, NF-κB and iNOS expression mRNA in spinal cord. CCI significantly increased the expression of NR2B (a) NF-κB (b) and iNOS(c) mRNA in the spinal cord, whereas DEX injection inhibited the upregulation of these mRNAs. Data shown as mean ± SD. *P < 0.05 versus sham group; #P < 0.05 versus CCI group.
