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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Trends Genet. 2017 Mar 31;33(5):336–348. doi: 10.1016/j.tig.2017.03.001

Figure 1.

Figure 1

SF3B1 and U2AF1 domains and functions in pre-mRNA splicing. (A) SF3B1 (top, yellow) and U2AF1 (bottom, cyan) domains and positions of mutational “hotspots” (red) [115, 116]. N626 only recently was classified as statistically significant hotspot across tumors [116] and is shown in lighter font. Mutation of R1074 (green) confers pladienolide-resistance. ULM, U2AF ligand motif; p14, p14-binding site; HR, HEAT repeat motif; ZnK, zinc knuckle motif; UHM, U2AF homology motif; RS/Gly-rich, arginine-serine-rich repeats with poly-glycine stretch. (B) SF3B1 and U2AF1 roles in the early stages of spliceosome assembly at the 3′ splice site. snRNP, small nuclear ribonucleoprotein particle; BPS, branch point sequence; ESE, exonic splicing enhancer; Py, polypyrimidine; AG, adenosine-guanosine consensus sequence preceding the 3′ splice site (SS).