Figure 2. ALT-803 mediates proliferation of NK cells from ascites of ovarian cancer patients.
PBMCs from healthy donors or ascites cells from ovarian cancer patients were labeled with CellTrace Violet dye and placed in culture for 1 week with media alone (NT), 1 nM ALT-803 (ALT) or 1 nM IL-15. Cells were then harvested and stained for surface antigens. (A) Representative histograms showing CellTrace dilution on healthy PBNK or patient ASC NK treated with ALT-803. (B) Pooled data showing proliferation (% cells that have diluted dye) on PBNK and ASC NK. Circles and squares represent different individuals within groups (n = 5 for PBNK and n =7 for ASC NK). One-way ANOVA was used to calculate statistics within the PBNK and ASC NK groups.