Fig. 3.
Preamplification increases the detection sensitivity MiR-15/107 TLDA analysis of cDNA template generated from the same RT product with or without preamplification are shown. The Cq of most miRNAs from the templates without preamplification (red dots and line) in miR-15/107 TDLA cards fell to the background (gray dots and line) level (Cq = 35); in contrast, preamplification array (brown dots and line) improved detection by several Cq values