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. 2017 May 30;8:620. doi: 10.3389/fimmu.2017.00620

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Copyright © 2017 Johdi, Ait-Tahar, Sagap and Jamal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peripheral blood mononuclear cells from the patients were stained with CD4, CD25, and CD127, and then analyzed on FACS Aria II (BD Biosciences). (A,B) Gating strategies used to sort CD4⁺CD25⁺CD127^low Tregs and CD4⁺CD25⁺CD127^high (non-Tregs). (C) Postsort purity determination from fraction 1. (D,E) FoxP3 staining analysis of the postsort CD4⁺CD25⁺CD127^low Tregs. (D) Fluorescence minus one staining. (E) FoxP3 staining on postsorted cells.