Figure 1.

Different enzyme-linked immunosorbant assay (ELISA) strategies for GH detection using specific nanobodies. (A) Schematic representation of the different methods used for detecting immobilized GH by free (I) or phage-Nb (II). Sandwich ELISA was designed for detecting GH (III and IV), phage-Nb (V), and phages (VI). Detection of phages was always performed using anti-M13-HRP antibody. (B) Detection of the immobilized (1 µg/ml) or NbGH04-captured GH (0.2 µg/ml) using five different anti-GH phage-Nb (10¹⁰ phages/ml). Significant values (*P < 0.01) comparing with their respective controls were marked with * on this graph and the following ones.