Figure 4.

Evaluation of the specificity and sensitivity of M13-NbGH07 toward GH. Enzyme-linked immunosorbant assay (ELISA) was performed using phage-Nbs, M13-NbGH01 and M13-NbGH07, and specific anti-GH and anti-GFP antibodies (1:3,000) against a directly immobilized antigens [1 µg/ml (A)], GH, TEV-GH, GFP-GH, or GFP, or after a capturing step with NbGH04 [0.2 µg/ml (B)]. (C) Detection sensitivity of M13-NbGH07 (10¹⁰ phages/ml) was tested in sandwich ELISA using different capturing agents (0.2 µg/ml); camel-anti-GH, rabbit-anti-GH, NbGH04, and NbGH06, in the presence of serial decimal concentrations of GH (nanograms per milliliter).