Figure 5.

Heat and UV stability tests of M13-NbGH07. NbGH07 (free, biotinylated, and phage-Nb), free NbGH04, and rabbit anti-GH were incubated for 0.5 h at different temperatures (A), at 90°C for different times (B) or exposed to different doses (joules) of UV^254nm (C). Treated samples were tested in sandwich enzyme-linked immunosorbant assay (ELISA) for capturing (Model III) or detecting (Model I) of GH. The integrity of treated phage-Nb of NbGH07 was tested using sandwich ELISA (model VI). Insets in panels (A,C) are for LB agar plates (with ampicillin) showing streaks of TG1 cells infected with the phage-Nb particles after exposure to the different temperatures (for 0.5 h) or UV^254nm doses.