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. 2017 May 30;8:115. doi: 10.3389/fendo.2017.00115

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Copyright © 2017 Murad, Assaad, Al-Shemali and Abbady.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitation of blood content of GH using M13-NbGH07 phage enzyme-linked immunosorbant assay (ELISA). GH content (nanograms per milliliter) was measured in five plasma samples using phage (model IV) or conventional (model III) sandwich ELISA. Absorbance values were calculated using the linear equations of the standard curve of known GH concentrations resulted from the two different ELISA models (inset). GH concentration values in the plasma samples were determined using a commercial ELISA test (Kit).