Figure 3.

miR-193b-3p directly targets TSC1. (A) Sequence analysis of miR-193b-3p mature miRNA binding with the 3′-UTR of TSC1. (B) Luciferase reporter assay shows a reduction of luciferase activity in NSC-34 cells with wild-type TSC1 3′UTR (luc-TSC1-WT) plasmids. The results were averages of three independent experiments. Data represent the mean ± SEM. **P < 0.01 vs. controls. (C) qRT-PCR results indicate the mRNA levels of TSC1 in NSC-34 cells transfected with miR-193b-3p mimics, inhibitors or scrambled sequence (100 nM, respectively). The results were averages of four independent experiments. Data represent the mean ± SEM. *P < 0.5 and **P < 0.01 vs. controls. miR-NC, miRNA negative control.