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. 2017 May 30;10:160. doi: 10.3389/fnmol.2017.00160

Figure 4.

Figure 4

miR-193b-3p activates mTOR complex 1 (mTORC1) signaling by decreasing TSC1 in NSC-34 cells. (A–D) Western blots and quantification to show the protein levels of TSC1/2 and mTORC1 indicators (pp70S6K and p4EBP1) in NSC-34 cells transfected with miR-193b-3p mimics, inhibitors or scrambled sequence. The results were averages of four independent experiments. Data represent the mean ± SEM. *P < 0.5 and **P < 0.01 vs. controls. (E,F) Western blots and quantification to show the protein levels of mTORC1 indicators (pp70S6K and p4EBP1), TSC1/2, p62 and LC3 in the spinal cord of SOD1G93A mutants compared with controls. The results were averages of three pairs of littermate mice. Data represent the mean ± SEM. *P < 0.05 vs. controls.