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. 2017 May 30;8:918. doi: 10.3389/fpls.2017.00918

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Copyright © 2017 Halder, Upadhyaya and Ray.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT PCR analysis carried out in Sorghum bicolor for relative expression of SbDhn1 under different stress conditions at different time points (0, 12, 24, and 48 h) where the EIF1 gene was used as an internal control to normalize the expression level. (A) High temperature (48°C), (B) osmotic stress generated by supplementing 200 mM sorbitol in MS medium, and (C) low temperature (4°C). Expression levels were statistically analyzed against unstressed plants using student’s t-test (^∗∗∗ P < 0.0001). Data shown are illustrative of at least three independent experiments.