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. 2017 May 30;8:918. doi: 10.3389/fpls.2017.00918

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Copyright © 2017 Halder, Upadhyaya and Ray.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurements of different physiological parameters of SbDhn1transformed tobacco plants along with wild-type and empty vector transformed plants after 21 days of osmotic stress generated by supplementing 200 mM of sorbitol in MS medium. Unstressed wild-type plant served as a control in this experiment. (A) Total chlorophyll content (B) soluble sugar content (C) relative electrolytic leakage (D) malonyl dialdehyde (MDA) content (E) relative water loss. Error bar represents ± SEM from at least three experimental replicates. All data were statistically analyzed against unstressed plants using student’s t-test (^∗∗∗ P < 0.0001, ^∗∗ P < 0.001). Data shown are illustrative of at least three independent experiments.