Figure 4.

Relative mRNA expression levels of in vivo isolated CD4⁺CD25⁺ cells from CD6^−/− and CD6^+/+ mouse spleens following direct T-cell receptor complex cross-linking. Sorted CD4⁺CD25⁺ T cells [regulatory T cells (T_reg)] from CD6^−/− (n = 5, solid bars) and CD6^+/+ (empty bars) were stimulated in anti-CD3 (1 µg/mL) coated plates. Relative mRNA expression for the indicated genes was measured after 7 h of stimulation by real-time PCR. n.s., not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (Student’s t-test).