Figure 8.

In vitro proliferative responses of B220⁺ cells from CD6^−/− and CD6^+/+ mice to IgM cross-linking and lipopolysaccharide (LPS) stimulation. (A) Negatively selected spleen B cells from CD6^−/− and CD6^+/+ mice were CFSE-labeled and cultured for 3 days either unstimulated (w/o stimulus) or stimulated with F(ab′)₂ anti-IgM (αIgM; 10 µg/mL) alone or in the presence of anti-CD40 (αCD40; 5 µg/mL) or IL-4 (10 ng/mL). (B) Same cells as in (A) were stimulated for 3 days with LPS (20 µg/mL) alone or plus IL-4 (30 ng/mL). Histograms show the CFSE fluorescence intensity of cells from a representative experiment of two performed.