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. 2017 May 30;11:152. doi: 10.3389/fncel.2017.00152

Figure 5.

Figure 5

More Kv1.1 and Kv1.2 cofractionate in the PSD with mTORC1 inhibition in vivo. (A) Representative Western blots depicting increased levels of Kv1.1 and Kv1.2 in the PSD fraction (Triton X-100 insoluble) of synaptoneurosomes with mTORC1 inhibition by rapamycin (R). DMSO (D) served as control. Total protein as measured by densitometry of Ponceau-S staining was used to normalize Kv1.1 and Kv1.2 protein expression. (B) In the soluble fraction (Triton X-100 soluble) of synaptoneurosomes, only Kv1.1 protein was increased with rapamycin. Tubulin was used to normalize Kv1.1 and Kv1.2 protein levels. (C) Quantification (average ± SEM) is expressed as densitometric ratio between protein values in rapamycin-treated and DMSO-treated rats. Kv1.1 (PSD = 1.31 ± 0.07; Soluble = 1.39 ± 0.13). Kv1.2 (PSD = 1.39 ± 0.08; Soluble = 0.92 ± 0.09). N = 3 animals per condition. Statistics: Student’s t-test; *p < 0.05.