Figure 5.

Presence of gsm genes and capsular polysaccharides production in M. feriruminatoris isolates. Gsm genes identified in the type strain G5847, namely gsm3-9 and gsm6-20, were detected (+) or not (−) by two specific PCRs. Capsular polysaccharide (CPS) were purified from 8 to 24 h cultures and identified by dot-blots revealed by Periodic acid–Schiff (PAS) staining (general polysaccharides detection) and antisera targeting galactan and anti β-(1→6)-glucan. At 24 h, the purified CPS were further quantified (μg of glucose equivalent per ml of culture) and 1 μg was deposited on the nitrocellulose membrane.