FIGURE 4.

Streptomyces albus-pAPI growth curves (solid lines) and apigenin production curves (dashed lines) for three different types of cultivation experiments (mean values from three independent triplicate flasks). Green colors correspond to control cultures with 10⁷ spores/mL. Blue colors correspond to conditioned cultures with a preinoculum of 10⁵ spore equivalents/mL. Orange colors correspond to conditioned cultures with a preinoculum of 10⁶ spore equivalents/mL. Apigenin production is anticipated in conditioned cultures, even with a delayed growth during the first 24-h, and achieving same production levels 1 and 2 days in advance, respectively. Maximum production levels for apigenin titers are labeled by arrows.