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. 2017 May 30;8:935. doi: 10.3389/fmicb.2017.00935

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Copyright © 2017 Freudenberger Catanzaro, Champion, Mohapatra, Cecere and Inzana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophoretic profile of large molecular size F. novicida extracts. Extracts from F. novicida were separated by electrophoresis through a 4–12% SDS-PAGE gel. The gels were subsequently stained by silver stain (A), Stains-All (B), and Stains-All/silver (C). LVS CLC was used for comparison. Analysis of the profiles focused on material >100 kDa. Lanes: M, molecular size standards; 1, LVS crude CLC in 1 M urea; 2, F. novicida crude extract in 1 M urea; 3 F. novicida soluble fraction extract (urea extracted); 4, F. novicida insoluble fraction extract (urea extracted); 5, F. novicida soluble fraction extract (phenol extracted).