Table 1.

Bacterial strains used in this study.

Strain/Plasmid	Description	Source
F. novicida	U112 wild type strain	Dr. Karen Elkins
F. novicida_P10	F. novicida subcultured 10 times in CDMB	This work
F. novicida Δ1212–1218	F. novicida with a deletion spanning FTN_1212 through FTN_1218; insertion of kanamycin resistance cassette	This work
F. novicida Δ1212–1218_P10	F. novicida Δ1212–1218 subcultured 10 times in CDMB	This work
tnfn1_pw060323p05q162	F. novicida strain with a T20 transposon insertion in FTN_1212	Gallagher et al., 2007
tnfn1_pw060323p03q152	F. novicida strain with a T20 transposon insertion in FTN_1213	Gallagher et al., 2007
tnfn1_pw060328p06q149	F. novicida strain with a T20 transposon insertion in FTN_1214	Gallagher et al., 2007
tnfn1_pw060323p05q110	F. novicida strain with a T20 transposon insertion in FTN_1215	Gallagher et al., 2007
tnfn1_pw060420p04q184	F. novicida strain with a T20 transposon insertion in FTN_1216	Gallagher et al., 2007
tnfn1_pw060418p03q107	F. novicida strain with a T20 transposon insertion in FTN_1217	Gallagher et al., 2007
tnfn1_pw060323p07q127	F. novicida strain with a T20 transposon insertion in FTN_1218	Gallagher et al., 2007
Type A F. tularensis strain TI0902	Type A wildtype strain	Inzana et al., 2004
LVS	F. tularensis subspecies holarctica live vaccine strain	Dr. May Chu
LVS_P10	LVS subcultured 10 times in CDMB	Bandara et al., 2011
E. coli DH5α	Genotype: F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(${\text{r}}_k^{-}$, ${\text{m}}_k^{+}$) phoAsupE44 thi-1 gyrA96 relA1 λ−	Invitrogen
E. coli Top10	Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG	Invitrogen
pSC-1423/1422K	Francisella suicide vector containing flanking regions to FTL_1423-22 with kanamycin resistance	Bandara et al., 2011
pMP822	Hygromycin resistant E. coli/Francisella shuttle vector with a blab promoter	LoVullo et al., 2009
pMP_FTN1212-1213	pMP822 with FTN1212-1213 inserted downstream of the blab promoter	This work