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. 2017 May 30;8:973. doi: 10.3389/fmicb.2017.00973

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Copyright © 2017 Chen, Duan, Zhou, Yu, Zhu, Cao and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The growth of Pst DC3000 was determined by qPCR-based biomass. Disease progression of on M. truncatula plants inoculated with Pst DC3000 and/or S. meliloti Sm2011 at 3, 5, and 8 dpi. For qPCR analysis about 30 ng of DNA were mixed with 0.4 mM gene specific primers [bacterial biomass: oprf gene (Ross and Somssich, 2016), plant biomass: actin]. Normalized plant DNA of actin and normalized pathogen DNA of oprf, pathogen or plant DNA is related to plant biomass. The error bars indicate standard deviations of three independent biological replicates. Different letters indicate significant differences as determined by a t-tests (p ≤ 0.05).