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. 2017 May 30;8:372. doi: 10.3389/fphys.2017.00372

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Copyright © 2017 Mitsiadis, Catón, Pagella, Orsini and Jimenez-Rojo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of Notch proteins in the injured dental pulp. (A) Schematic illustration showing dental pulp injury after cavity preparation. Arrowheads indicate the areas of Notch activation within the pulp. (B) Notch1 (N1) staining is visible in cells lining blood vessels within the central part of the pulp. (C) Notch3 (N3) labeling is detected in perivascular cells of the central part of the pulp. (D) Notch2 (N2) staining is mainly found in apical pulp cells at the tooth apex. (E,F) Whole mount immunostaining showing Notch2 protein distribution at the apical part of an injured pulp. Scale bars: 50 μm (B–D), 200 μm (E,F).